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TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

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Life Science Buffers

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

Ethanol, Absolute (200 Proof), Molecular Biology Grade, Fisher BioReagents™

CAS: 64-17-5 Molecular Formula: C2H6O InChI Key: LFQSCWFLJHTTHZ-UHFFFAOYSA-N Synonym: ethyl alcohol,alcohol,methylcarbinol,grain alcohol,ethyl hydroxide,ethyl hydrate,algrain,alcohol,anhydrol,tecsol PubChem CID: 702 ChEBI: CHEBI:16236 IUPAC Name: ethanol SMILES: CCO

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Ethanol

PubChem CID	702
CAS	64-17-5
ChEBI	CHEBI:16236
SMILES	CCO
Synonym	ethyl alcohol,alcohol,methylcarbinol,grain alcohol,ethyl hydroxide,ethyl hydrate,algrain,alcohol,anhydrol,tecsol
IUPAC Name	ethanol
InChI Key	LFQSCWFLJHTTHZ-UHFFFAOYSA-N
Molecular Formula	C2H6O

Thermo Scientific™ DNA AWAY™ Surface Decontaminant

Eliminate unwanted DNA and DNase from glassware and plasticware without affecting subsequent DNA samples. This surface decontaminant degrades DNA more quickly and effectively than autoclaving.

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Laboratory Disinfectants

Dimethyl Sulfoxide, Fisher BioReagents™

CAS: 67-68-5 Molecular Formula: C2H6OS Molecular Weight (g/mol): 78.13 MDL Number: MFCD00002089 InChI Key: IAZDPXIOMUYVGZ-UHFFFAOYSA-N Synonym: dimethyl sulfoxide,dmso,methyl sulfoxide,dimethylsulfoxide,dimethyl sulphoxide,methane, sulfinylbis,demsodrox,demasorb,demavet,dimexide PubChem CID: 679 ChEBI: CHEBI:28262 IUPAC Name: methylsulfinylmethane SMILES: CS(C)=O

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Dimethyl Sulfoxide

PubChem CID	679
CAS	67-68-5
Molecular Weight (g/mol)	78.13
ChEBI	CHEBI:28262
MDL Number	MFCD00002089
SMILES	CS(C)=O
Synonym	dimethyl sulfoxide,dmso,methyl sulfoxide,dimethylsulfoxide,dimethyl sulphoxide,methane, sulfinylbis,demsodrox,demasorb,demavet,dimexide
IUPAC Name	methylsulfinylmethane
InChI Key	IAZDPXIOMUYVGZ-UHFFFAOYSA-N
Molecular Formula	C2H6OS

Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Life Science Buffers

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Invitrogen™ SuperScript™ III Reverse Transcriptase

Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.

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Reverse Transcriptases

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Reverse Transcriptase	SuperScript III
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Reaction Speed	30 to 50 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

Dichloromethane, 99.8+%, for analysis, stabilized with amylene, Thermo Scientific Chemicals

CAS: 75-09-2 Molecular Formula: CH2Cl2 Molecular Weight (g/mol): 84.93 MDL Number: MFCD00000881 InChI Key: YMWUJEATGCHHMB-UHFFFAOYSA-N Synonym: methylene chloride,methylene dichloride,methane, dichloro,methylene bichloride,methane dichloride,solaesthin,solmethine,freon 30,narkotil,aerothene mm PubChem CID: 6344 ChEBI: CHEBI:15767 IUPAC Name: dichloromethane SMILES: ClCCl

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Methylene Chloride

PubChem CID	6344
CAS	75-09-2
Molecular Weight (g/mol)	84.93
ChEBI	CHEBI:15767
MDL Number	MFCD00000881
SMILES	ClCCl
Synonym	methylene chloride,methylene dichloride,methane, dichloro,methylene bichloride,methane dichloride,solaesthin,solmethine,freon 30,narkotil,aerothene mm
IUPAC Name	dichloromethane
InChI Key	YMWUJEATGCHHMB-UHFFFAOYSA-N
Molecular Formula	CH2Cl2

Water, Molecular Biology Grade, Fisher BioReagents

CAS: 7732-18-5 Molecular Formula: H2O Molecular Weight (g/mol): 18.015 InChI Key: XLYOFNOQVPJJNP-UHFFFAOYSA-N Synonym: dihydrogen oxide,dihydrogen monoxide PubChem CID: 962 ChEBI: CHEBI:15377 IUPAC Name: oxidane SMILES: O

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Molecular Biology Grade Life Science Water

PubChem CID	962
CAS	7732-18-5
Molecular Weight (g/mol)	18.015
ChEBI	CHEBI:15377
SMILES	O
Synonym	dihydrogen oxide,dihydrogen monoxide
IUPAC Name	oxidane
InChI Key	XLYOFNOQVPJJNP-UHFFFAOYSA-N
Molecular Formula	H2O

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

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Life Science Buffers

Content And Storage	Room temperature
Form	Liquid

Invitrogen™ TE Buffer

This 1X TE Buffer is a component of the PureLink™ 96 Plasmid Purification System, now offered separately.

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DNA Extraction and Purification

Ampicillin Sodium Salt (Crystalline Powder), Fisher BioReagents™

CAS: 69-52-3 Molecular Formula: C16H21N3NaO4S Molecular Weight (g/mol): 374.411 InChI Key: BSFVNXCYXDYHOD-ZQDFAFASSA-N Synonym: Ampicillin PubChem CID: 131673879 IUPAC Name: (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;molecular hydrogen;sodium SMILES: [HH].CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C.[Na]

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Antibiotics

PubChem CID	131673879
CAS	69-52-3
Molecular Weight (g/mol)	374.411
SMILES	[HH].CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C.[Na]
Synonym	Ampicillin
IUPAC Name	(2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;molecular hydrogen;sodium
InChI Key	BSFVNXCYXDYHOD-ZQDFAFASSA-N
Molecular Formula	C16H21N3NaO4S

Agarose (Low-EEO/Multi-Purpose/Molecular Biology Grade), Fisher BioReagents™

CAS: 9012-36-6 Molecular Formula: C₁₂H₁₈O₉

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Electrophoresis Gel Casting Reagents

CAS	9012-36-6
Molecular Formula	C₁₂H₁₈O₉

Hydrochloric Acid, 37%, Certified AR for Analysis, d=1.18, Fisher Chemical™

CAS: 7647-01-0 Molecular Formula: ClH InChI Key: VEXZGXHMUGYJMC-UHFFFAOYSA-N PubChem CID: 313 ChEBI: CHEBI:17883

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Hydrochloric Acid

PubChem CID	313
CAS	7647-01-0
ChEBI	CHEBI:17883
InChI Key	VEXZGXHMUGYJMC-UHFFFAOYSA-N
Molecular Formula	ClH

Thermo Scientific Chemicals Paraformaldehyde, 4% in PBS

CAS: 30525-89-4 Molecular Formula: CH2O Molecular Weight (g/mol): 30.026 MDL Number: MFCD00133991 InChI Key: WSFSSNUMVMOOMR-UHFFFAOYSA-N Synonym: formalin,methanal,formol,methylene oxide,paraformaldehyde,oxomethane,paraform,formic aldehyde,oxymethylene,methyl aldehyde PubChem CID: 712 ChEBI: CHEBI:16842 IUPAC Name: formaldehyde SMILES: C=O

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Histology Reagents

PubChem CID	712
CAS	30525-89-4
Molecular Weight (g/mol)	30.026
ChEBI	CHEBI:16842
MDL Number	MFCD00133991
SMILES	C=O
Synonym	formalin,methanal,formol,methylene oxide,paraformaldehyde,oxomethane,paraform,formic aldehyde,oxymethylene,methyl aldehyde
IUPAC Name	formaldehyde
InChI Key	WSFSSNUMVMOOMR-UHFFFAOYSA-N
Molecular Formula	CH2O

Invitrogen™ dNTP Set (100 mM)

Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.

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dNTPs

Shipping Condition	Approved for shipment on Wet or Dry Ice
Form	Liquid
Label or Dye	Unlabeled
Concentration	100 mM
For Use With (Application)	Standard PCR,RT-PCR,Real Time PCR (qPCR)

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