Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Gibco™ PBS, pH 7.2
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PBS, pH 7.2
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 280 to 320 mOsm/kg |
Gibco™ PBS (10X), pH 7.4
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This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS (10X), pH 7.4
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.3 to 7.5 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3100 mOsm/kg |
TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents
Color | Colorless |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Tris-EDTA |
Grade | Molecular Biology |
DNase | DNase free |
Filtered Through | Filtered through a 5-micron filter. |
ChemAlert Storage Symbol | Gray |
Name Note | 1X Solution, pH 8.0 |
CAS | 7732-18-5 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Health Hazard 2 | CAUTION! |
pH | 7.4 to 8.1 |
Synonym | TE |
Recommended Storage | RT |
Gibco™ PBS (10X), pH 7.2
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS (10X), pH 7.2
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Capacity | 500 mL |
pH | 7.1 to 7.3 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2800 to 3200 mOsm/kg |
Gibco™ PBS, pH 7.4 (flow cytometry grade)
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of applications, including reagent preparation, diluting cells for flow cytometry and as a cell culture reagent.
Medicago Phosphate Buffered Saline Tablets, pH 7.4
Specifically developed for immunological and microbiological laboratories. Medicago Phosphate Buffered Saline Tablets, pH 7.4 are provided as pre-weighed tablets with a final volume of 500 or 1000ml per tablet.
Content And Storage | 25°C |
---|---|
Form | Tablet |
Color | White |
pH | 7.4 |
Grade | Analytical Grade |
For Use With (Application) | Immunoassays, tissue and cell culture procedures, immuno-histochemical procedures, microbiological procedures, and sample dilution |
Tris Buffered Saline Tablets, pH 7.6, Medicago
Supplied as exactly pre-weighed tablets. Medicago Tris Buffered Saline Tablets, pH 7.6 gives 500ml of 1x solution with 0.05M Tris-HCl buffer and 0.15M sodium chloride. Manufactured in ISO 9001 and 13485 certified facilities.
Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free
PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.
Shipping Condition | Room Temperature |
---|---|
Dilution | 10 X |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.4 |
Product Line | Ambion™ |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Ambient |
---|---|
Dilution | 1 X |
Content And Storage | 15°C to 30°C |
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
pH | 7.3 to 7.5 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Gibco™ PBS, pH 7.4
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Dilution | 1 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.4
Non-toxic to most cells and is used in biological research.
Dilution | 10 X |
---|---|
Form | Liquid |
Product Type | Phosphate Buffered Saline (PBS) |
Color | Colorless |
pH | 7.4 |
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00132476 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 8.0
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
PubChem CID | 23831 |
---|---|
CAS | 7365-45-9 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
MDL Number | MFCD00006158 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Molecular Formula | C8H18N2O4S |
Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
Health Hazard 3 | P264b-P280-P302+P352-P305+P351+P338-P332+P313-P362 |
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Solubility Information | It is soluble in water. |
Packaging | Plastic bottle |
Physical Form | Liquid |
Health Hazard 1 | H315-H319-H335 |
Chemical Name or Material | TRIS-Tricine-SDS running buffer (10X) |
TSCA | Yes |
Recommended Storage | Ambient temperatures |