Gel Electrophoresis Equipment
Gel Electrophoresis Equipment
Tank assemblies and components for acrylamide, agarose, and IEF electrophoresis of proteins, nucleic acids, and peptides; includes assembly, operation, gel casting, drying, maintenance, and imaging components, replacement parts, and accessories.
Gel electrophoresis is the preferred technique for separating the components of samples that contain nucleic acid (DNA and RNA) and protein macromolecules.
The procedure is performed by placing the sample(s) at one end of an electrophoresis gel in small wells or indentations. An electrical source is attached and runs for a specified timed. A buffer provides ions to help carry the current and maintain pH.
The molecules move based on electrical charge. Proteins are pre-treated with the detergent sodium dodecyl sulfate (SDS) to denature and coat it with a negative charge. Molecules travel at a speed inversely related to size or length, and the degree of separation (distance between the bands) depends on the gel concentration.
Separations usually include electrophoresis(also called DNA ladders or protein ladders) that contain molecules of known sizes and are used for comparison. A variety of buffers and other electrophoresis reagents are also part of the process.
Electrophoresis gels are typically agarose or polyacrylamide; each is suited to different molecules. Polyacrylamide gel electrophoresis (PAGE) is usually used for proteins and smaller DNA fragments. Polyacrylamide gels form by chemical reaction and have uniform pore sizes. They can be cast by the user or purchased as ready-to-use or pre-cast gels.
Agarose gels contain natural polysaccharide polymers from seaweed and have irregular pore sizes. They work well for separating proteins larger than 200 kDa or DNA fragments from 50 to 20,000 bp. Pre-cast agarose gels are available, as are powdered agarose gels for on-site casting.
Polyacrylamide gels are typically run vertically, while agarose gels are run in a horizontal position. Vertical and horizontal electrophoresis equipment is widely available, along with a variety of casting chambers and other electrophoresis system accessories.
Cytiva Immobiline™ DryStrip Kit and Components
Precast Immobiline DryStrip gels for running the first dimension of a 2-D electrophoresis experiment with the Multiphor II electrophoresis system
Cytiva Immobiline™ DryPlate for IEF
Obtain reproducible IEF separations with dry, precast gels containing immobilized pH gradients
Cytiva EPS 301 Electrophoresis Power Supply
Cover the wide range of electrophoresis and blotting applications. Designed for performance, EPS Power Supplies can be relied on for security and reproducibility
Cytiva IPGbox
Tools for enhancing reswelling of precast Immobiline DryStrip gels (IPG strips)
Cytiva Accessories For Multiphor™ II Flatbed Electrophoresis System: Cooling Plate
Accessories For Multiphor™ II Flatbed Electrophoresis System
Cytiva IPGbox Kit
Tools for enhancing reswelling of precast Immobiline DryStrip gels (IPG strips)
Cytiva Power Supply Adapters
Connecting 4 mm or 2 mm leads to 2 mm or 4 mm output jacks, respectively
Cytiva EPH/IEF Electrodes for Multiphor™ II Flatbed Electrophoresis System
For use with Multiphor™ II flatbed electrophoresis system
Promotions are available
Cytiva EPS 3501 High Voltage Power Supplies for Electrophoresis Units
Cover the wide range of electrophoresis and blotting applications. Designed for performance, EPS Power Supplies can be relied on for security and reproducibility
Promotions are available
Cytiva Glass Plate
Glass plate for use with the miniVE Vertical Electrophoresis System.
Promotions are available
Cytiva SE260/SE250 MiniVertical Electrophoresis System Replacement Parts: Plates
Promotions are available
Cytiva Accessories For Multiphor™ II Electrophoresis Systems
Accessories for analytical or preparative separations of proteins and peptides by isoelectric focusing (IEF), native or SDS PAGE, 2-D PAGE and DNA electrophoresis
Promotions are available