Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.

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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

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Pricing and Availability
Specifications

Sensitivity	Low picogram to mid-femtogram
Form	Liquid
Product Type	Substrate
Membrane Compatibility	Nitrocellulose, PVDF
Product Line	Pierce™
Detection Method	Chemiluminescence, Fluorescence

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

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Pricing and Availability
Specifications

Substrate	Horseradish Peroxidase
Form	Liquid
Product Type	Substrate
Signal Duration	6 to 8 hr.
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

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Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Substrate	Horseradish Peroxidase
Recommended Antibody Concentrations	2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Conjugate	HRP
Form	Liquid
Product Type	Substrate
Signal Duration	6 to 24 hr.
For Use With (Application)	Western Blot
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

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Pricing and Availability
Specifications

Substrate	Horseradish Peroxidase
Content And Storage	Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form	Liquid
Product Type	Substrate
Signal Duration	1 to 2 hr.
Product Line	Pierce™
Detection Method	Chemiluminescence

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

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Pricing and Availability
Specifications

Shipping Condition	Wet Ice
Substrate	Horseradish Peroxidase
Sensitivity	Low-femtogram to high-attogram
Form	Liquid
Product Type	Substrate
Membrane Compatibility	Nitrocellulose, PVDF
For Use With (Application)	Western Blot
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

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Pricing and Availability
Specifications

Substrate	Horseradish Peroxidase
Sensitivity	Mid-femtogram
Form	Liquid
Product Type	Substrate
Signal Duration	24 hr.
Product Line	SuperSignal™
Detection Method	Chemiluminescence

Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent

Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.

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Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent

Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.

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Pricing and Availability
Specifications

Content And Storage	2°C to 8°C
Sensitivity	∼1 pg
Product Type	ECL Western Blot Detection Reagent
For Use With (Application)	Sensitive Quantitative or Confirmatory Western Blot

Merck Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Pricing and Availability
Specifications

Substrate	Horseradish Peroxidase
Content And Storage	2°C to 8°C
Shelf Life	9 Months
For Use With (Application)	Dot/Slot Blot

Cytiva Amersham™ ECL™ Western Blotting Detection Reagents

Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.

Pricing and Availability
Specifications

Content And Storage	2°C to 8°C
Sensitivity	∼10 pg
Product Type	ECL Western Blot Detection Reagent
For Use With (Application)	Western Blot

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

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Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

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