Direct PCR Reagents and Kits
Direct PCR Reagents and Kits
- (16)
- (3)
- (27)
- (3)
- (10)
- (1)
- (2)
- (8)
- (1)
- (2)
- (1)
- (2)
- (23)
- (5)
- (1)
- (1)
- (11)
- (1)
- (2)
- (1)
- (2)
- (2)
- (36)
- (9)
- (11)
- (39)
- (11)
- (2)
- (30)
- (2)
- (6)
- (6)
- (14)
- (11)
- (1)
Filtered Search Results
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Format | Spin Column |
---|---|
Run Time | 5 min. |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Thermo Scientific™ Phire Plant Direct PCR Master Mix
Amplify DNA directly from plant samples without prior DNA purification with Phire Plant Direct PCR Master Mix
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Plant |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping |
PCR Method | Direct PCR |
Product Line | Phire |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Amplify DNA directly from variety of human and animal samples with no prior purification with Phire Tissue Direct PCR Master Mix.
Polymerase | Phire Hot Start II DNA polymerase |
---|---|
GC-Rich PCR Performance | Medium |
Includes Label or Dye | Yes |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Master Mix |
Sample Type | Tissue |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Genotyping |
PCR Method | Direct PCR |
Product Line | Phire |
First Strand cDNA Synthesis Kit
Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.
Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit
ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.
Shipping Condition | Room Temperature |
---|---|
Target | DNA from Enzymatic Reactions (e.g. PCR) |
Content And Storage | The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly. |
Format | Kit |
Product Type | PCR Clean-Up Kit |
Sample Type | PCR Products |
Isolation Technology | ChargeSwitch™ Chemistry |
Run Time | 5 min |
For Use With (Application) | Next-Generation Sequencing (NGS),Cloning,Standard PCR |
High-throughput Compatibility | High-throughput Compatible |
Product Line | ChargeSwitch™ |
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
---|---|
Format | Tube |
Product Type | 1-Step qPCR kit |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Sample Type | Cell, RNA |
Reaction Speed | Fast or Standard |
Reagent Type | Cell Lysis |
Green Features | Fewer resources used and less waste, Less hazardous |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Product Line | Cells-to-CT™, TaqMan™ |
Detection Method | Primer-probe |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Shipping Condition | Room Temperature |
---|---|
Format | Kit |
Sample Type | PCR Products |
Isolation Technology | Silica Spin Column |
Run Time | 10 min. |
Invitrogen™ TaqMan™ Fast Advanced Cells-to-CT™ Kit
The TaqMan Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Green Features | Fewer resources used and less waste, Less hazardous |
Concentration | 2X |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | High-throughput Compatible |
Detection Method | Primer-probe |
Format | Tube |
Product Type | Fast Advanced Cells-to-Ct Kit |
Sample Type | Cell |
Reaction Speed | Fast |
Reagent Type | Cell Lysis |
Purification Target | Lysate |
Product Line | Cells-to-CT™ |
Applied Biosystems™ SNaPshot™ Multiplex Kit
The SNaPshot™ Multiplex Kit is part of the SNaPshot™ Multiplex System. The SNaPshot™ Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs).
Product Type | Multiplex Kit |
---|---|
For Use With (Equipment) | 3500 Series Genetic Analyzers, SeqStudio Flex Series Genetic Analyzers, SeqStudio Genetic Analyzer, 3730/3730xl Genetic Analyzers |
Sample Type | DNA |
Technique | SNP Genotyping (Fragment Analysis-Based), Primer Extension |
Product Line | SNaPshot™ |
Detection Method | Primer-probe |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Efficient PCR directly from different plant materials across species.
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
Shipping Condition | Room Temperature |
---|---|
Separation Range | 100 bp to 12 kb |
Content And Storage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
Starting Material Volume | 125 μL |
Isolation Technology | Silica Spin Column |
Elution Volume Max | 20 μL |
For Use With (Application) | Cloning,Standard PCR |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Target | DNA from Enzymatic Reactions (e.g. PCR) |
Format | Spin Column |
Product Type | PCR Micro Kit |
Run Time | 10 min. |
Elution Volume Min | 5 μL |
Product Line | PureLink™ |
Invitrogen™ Single Cell Lysis Kit
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
Shipping Condition | Dry Ice |
---|---|
Content And Storage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Reverse Transcriptase | M-MLV |
Size (Final Product) | 7 kb or less |
Final Product Type | cDNA |
Sample Type | Mammalian Cells |
Purification Time | 2 hr. |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Product Line | Ambion™, Cells-to-cDNA™ |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
Shipping Condition | Frozen, Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Content And Storage | • Express Lysis Solution (room temperature or 4°C) • Express ezDNase (–20°C) • SuperScript IV VILO Master Mix (–20°C) • SuperScript IV VILO No RT Control (–20°C) • TaqMan Fast Advanced Master Mix (4°C) |
Form | Liquid |
Isolation Technology | Direct cell lysis |
Kit Type | Direct Cell Lysis RT-qPCR |
Green Features | Less hazardous, less waste |
For Use With (Application) | Gene Expression |
High-throughput Compatibility | Yes |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Target | cDNA |
Format | Kit |
Sample Type | Cultured mammalian cells |
Reaction Speed | Fast |
Label or Dye | ROX |
Purification Target | RNA |
Shelf Life | 1 Year |
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
For Use With (Equipment) | 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
---|---|
Sample Type | Cells |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | RT-PCR |
Product Line | CellsDirect™, Platinum™, SuperScript™ |
Detection Method | Primer-probe |