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Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1
Dynabeads MyOne Streptavidin T1 beads are 1-μm monosized, monodispersed, superparamagnetic beads covalently coupled to streptavidin and intended for use in the isolation and handling of biotinylated nucleic acids, antibodies, and other biotinylated ligands.
Shipping Condition | Ambient Temperature |
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Content And Storage | Dynabeads MyOne Streptavidin T1 are supplied in PBS, pH 7.4, with 0.1% BSA and 0.02% NaN3 . Store at 2–8°C. |
Description | Recombinant streptavidin covalently coupled to Dynabeads |
Isolation Technology | Magnetic Beads |
Color | Brown |
Binding Property | 950–1,500 pmoles free biotin/mg beads |
Concentration | 10 mg/mL |
High-throughput Compatibility | Yes |
For Use With (Application) | Capture of Biotinylated Targets |
Size Distribution | CV <5% |
Ligand Type | Streptavidin |
Regulatory Status | For Research Use Only |
Target | Biotin |
Format | Beads in suspension |
Bead Type | Magnetic Polystyrene |
Product Type | Streptavidin Magnetic Beads |
For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
Sample Type | Any Sample Type |
Certifications/Compliance | ISO9001 and ISO13485 |
Shelf Life | 48 months from date of manufacture |
Species | Recombinant |
Product Line | Dynabeads™, MyOne™ |
Polysorbate 80, Thermo Scientific Chemicals
CAS: 9005-65-6 Synonym: Polyoxyethylene(20)sorbitan monooleate
CAS | 9005-65-6 |
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Synonym | Polyoxyethylene(20)sorbitan monooleate |
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
Format | Roll |
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Length (Metric) | 4 m |
Material | 100% Pure Nitrocellulose |
For Use With (Application) | Western blots, dot-blot assays, and other protein or nucleic acid methods |
Thermo Scientific™ CL-XPosure™ Film
Thermo Scientific CL-XPosure Film is an economically priced clear-blue X-ray film especially for detection and documentation of ECL-based western blots and other chemiluminescent protein or nucleic acid assay methods.
Content And Storage | Store at room temperature. |
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Product Type | X-Ray Film |
Quantity | 100 Sheets |
Product Line | CL-Xposure |
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Thermo Scientific™ Pierce™ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
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Form | Powder |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA, Immunohistochemistry |
Product Line | Pierce™ |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Content And Storage | Store desiccated at -20°C, protect from moisture |
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Product Type | NHS-Fluorescein |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane
Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.
Pore Size | 0.45 μm |
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Thermo Scientific™ TMTpro™ 16plex Label Reagent Set
The Thermo Scientific TMTpro 16plex Label Reagent Set enables multiplexing of up to 16 samples for multiplexed protein identification and quantitation using tandem mass spectrometry (MS/MS).
Workflow Step | Protein Labeling |
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Final Product Type | Peptide |
Product Type | Isobaric Label Reagent Set |
For Use With (Equipment) | Mass Spectrometer |
Label or Dye | Tandem Mass Tag™ (TMT) Labels |
Detection Method | Mass Spectrometry |
Product Line | TMT™ |
Cytiva PD MiniTrap™ G-25 Sample Preparation Columns
Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.
Thermo Scientific™ Ionic Detergent Compatibility Reagent for Pierce™ 660nm Protein Assay Reagent
Red-to-green (A660nm), ready-to-use, detergent- and reducing agent-compatible assay reagent to measure total protein concentration vs. protein standard.
Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.