Invitrogen™ Amyloid beta 40 Human ELISA Kit

Description

The Human Amyloid Beta 40 (Hu Abeta 40) ELISA quantitates Hu Abeta 40 in human tissue culture medium, tissue homogenate, and cerebrospinal fluid (CSF). The assay will exclusively recognize both natural and recombinant Hu Abeta 40. Principle of the method The Human Abeta 40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Easy-to-run sandwich ELISA
The Aβ40 ELISA kit is a solid phase sandwich enzyme-linked immuno-sorbent assay (ELISA). A monoclonal antibody specific for the NH2-terminus of human Aβ has been coated onto the wells of the microtiter strips provided. During the first incubation, standards of known human Aβ40 content, controls, and unknown samples are pipetted into the wells and co-incubated with a rabbit antibody specific for the COOH-terminus of the 1–40 Aβ sequence. Bound rabbit antibody is detected through use of a horseradish peroxidase-labeled anti-rabbit antibody. Samples are quantitated using a standard curve generated from the recombinant Aβ40 included with the kit.

Results typically in one day—rapid 4-hour incubation protocol
The kit comes with the capture antibody precoated to the wells, eliminating the need to coat the plate. No overnight incubation needed. The incubation steps can be performed in four hours.

Precision: <10% CV inter-assay and intra-assay
The kits are validated for precision to help ensure that results are consistent within a plate, between plates, and between lots.

Economical, removable wells
The precoated wells come as removable 8-well strips, allowing you to tailor the assay size to your needs. Two standard vials are provided to give you the option of reconstituting a new vial should you choose to not run the entire plate at once.

• Easy-to-run sandwich ELISA
• Results typically in one day—rapid four-hour incubation protocol
• Precision: <10% CV intra-assay and inter-assay
• Precoated, removable 8-well strips
• Rigorous validation
• Each ELISA kit is validated for sensitivity, specificity, and lot-to-lot consistency

This kit does not detect Aβ42 or Aβ43. See protocol insert for more information on validation.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

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