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CD3 Mouse anti-Human, BD IMag-DM, Clone: HIT3A, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 552593

468.64 EUR valid until 2025-12-31
Use promo code "25339" to get your promotional price.

Product Code. 15826838

  • 469.00 EUR / Each

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Description

Description

BD IMag™ Anti-Human CD3 Particles DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD3-bearing leukocytes using the BD IMagnet™. CD3 has been reported to be expressed on all mature T lymphocytes and on developing thymocytes.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ anti-human CD3 Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

CD3
Monoclonal
BD IMag-DM
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Affinity Purified
RUO
Primary
Store undiluted at 4°C
Flow Cytometry
HIT3A
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916
Mouse
5 mL
Immunology
Human
IgG2a κ
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