CD45R/B220 Rat anti-Mouse, BD IMag-DM, Clone: RA3-6B2, BD

Description

BD IMag™ anti-mouse CD45R/B220 Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD45R/B220-bearing leukocytes using the BD IMagnet™. CD45R/B220 is reportedly expressed on B lymphocytes at all stages from pro-B through mature and activated B cell. It is also has been reported to be found on abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R/B220 antigen is reportedly not on hematopoietic stem cells, plasma cells, resting T lymphocytes, or MHC-restricted CTL.

Leukocytes are labeled with BD IMag™ anti-mouse CD45R/B220 Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.