CD56 Mouse anti-Human, BD IMag-DM, Clone: NCAM16.2, BD

Description

BD IMag™ Anti-Human CD56 Magnetic Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of the CD56-bearing leukocytes using the BD™ IMagnet. CD56, also known as the neural cell adhesion molecule (N-CAM), is expressed in many different cell and tissue types. In normal donors, it is found on NK and NK-T cells. The NCAM16.2 mAb has been reported to cross-react with lymphocytes of the rhesus macaque. BD IMag™ Anti-Human CD56 magnetic particles have been reported to effectively separate CD56-bearing cells of rhesus macaque blood. For enrichment of NK cells without NK-T cells, the use of BD™ IMag Anti-Human CD3 Magnetic Particles - DM (Cat. no. 552593) are suggested for depleting T lymphocytes before separation of CD56-positive leukocytes.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ Anti-Human CD56 Magnetic Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™. Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications, such as by flow cytometry.