CD8a Rat anti-Mouse, BD IMag-DM, Clone: 53-6.7, BD

Description

BD IMag™ anti-mouse CD8a Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD8a-bearing leukocytes using the BD IMagnet™. CD8a has been reported to be expressed on most thymocytes and a subpopulation of mature T lymphocytes (e.g. MHC class I-restricted T cells, including most T suppressor/cytotoxic cells). In addition, subsets of γδ TCR-bearing T cells, intestinal intraepithelial lymphocytes, and dendritic cells also have been reported to express CD8a. Leukocytes are labeled with BD IMag™ anti-mouse CD8a Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetc field of BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The seperation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

Host Species:
Clone: 53-6.7

Cell Separation