CD90.2 Rat anti-Mouse, BD IMag-DM, Clone: 30-H12, BD

Description

BD IMag anti-mouse CD90.2 (Thy-1.2) Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD90.2-bearing leukocytes using the BD IMagnet™. The CD90.2 alloantigen is expressed on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains. 30-H12 mAb has been reported not to
cross-react with mouse Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1. Leukocytes are labeled with BD IMag™ anti-mouse CD90.2 Particles - DM according to the Magnetic Labeling Protocol. his labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

Host Species:
Clone: 30-H12

Cell Separation