Transferrin Receptor Mouse anti-Human, Clone: 236-15375, Invitrogen™

Description

The anti-human transferrin receptor antibody should be useful for the localization of transferrin receptors by immunofluorescence techniques. This antibody recognizes the human transferrin receptor and does not block binding of transferrin to the receptor. Although fluorescent transferrin conjugates have been widely used to study transferrin receptor-mediated endocytosis, antibodies to the transferrin receptor have proven to be useful for localizing transferrin receptors, studying endocytic recycling pathways and performing enzyme-linked immunosorbent assays (ELISAs). Upon receipt, store desiccated at -20°C. Stored properly, the antibody should remain stable for at least one year. To prepare a stock solution, reconstitute the antibody in PBS with 1% BSA and 2 mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4°C. For longer storage, divide into aliquots and freeze at -20°C.

CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as a homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin, a monomeric serum glycoprotein (MW∽80,000 daltons), and up to two Fe3+ atoms), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of the iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.