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MP Biomedicals™ Basal Medium Eagle (Modified), with Hanks' Salts and L-Glutamine, without Sodium Carbonate
Basal Medium Eagle (Modified), with Hanks' Salts and L-Glutamine, without Sodium Carbonate is one of the most commonly used synthetic basal medium for supporting the growth of many different mammalian cells.
Brand: MP Biomedicals™ 091003122
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Description
- Basal Medium Eagle (BME) is one of the most commonly used of all cell culture media.
- This medium is the result of numerous studies from the late 1950's analyzing the nutrient requirements and other components essential for the growth of cells in culture.
- Traditionally, BME has been used in studies to measure the growth response of normal (WI-38) and transformed (HeLa) cells in monolayer culture.
- BME may be used for a broad variety of cell lines when properly supplemented.
- It has a pH of 6.5 ± 0.5 (w/o sodium bicarb.), 7.3 ± 0.5 (adjusted with sodium bicarb.)
Specifications
| Powder | |
| Bacteria Cell Culture, Mammalian Cell Culture | |
| Suspend 42.5 g of the medium in one liter of distilled or deionized water. Mix well and heat with frequent agitation. Boil for one minute. Dispense and sterilize in an autoclave at 121°C (15 lb. psi) for 15 minutes. Cool to 45 to 50°C and add 5 to 10% sterile defibrinated sheep or rabbit blood if desired. |
| Polypeptone 10.0, Biotryptase 10.0, Heart Peptone 3.0, Corn Starch 1.0, Sodium Chloride 5.0, Agar 13.5, Final pH 7.3 ±0.2 | |
| 6.5 to 7.3 ±0.5 | |
| 10 L |
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.