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Gibco™ BenchStable™ DMEM/F12
Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.
Brand: Gibco™ A4192002
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Description
Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
BenchStable DMEM/F-12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM/F12 when supplemented with ≥10% fetal bovine serum (FBS).
This DMEM/F-12 is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPEPS
Using BenchStable DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).
Specifications
| Store at room temperature. Protect from light. | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate | |
| Energy efficient, Fewer resources used, Sustainable packaging | |
| BenchStable™ | |
| Room Temperature |
| MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
| Sterile-filtered | |
| No HEPES | |
| cGMP-compliant under the ISO 9001: 2015 standard | |
| 10 x 500 mL |
For Research Use Only. Not for use in diagnostic procedures.