beta-Arrestin 2 Polyclonal Antibody, Invitrogen™

Description

PA1-732 detects beta-Arrestin 2 in rat, human and bovine samples. PA1-732 has been successfully used in Western blot procedures. By Western blot, this antibody detects ∽49 kDa and ∽47 kDa proteins representing pan Arrestin in rat and bovine retina as well as rat brain samples. The PA1-732 immunogen is a synthetic peptide corresponding to residues C D(384) D I V F E D F A R L R L K(397) of human beta-arrestin 2. This peptide (Cat. # PEP-281) is available for use in neutralization and control experiments.

Beta-arrestin-2, or ARRB2, is an adaptor protein belonging to the arrestin family with an N- arrestin domain and a C- arrestin domain. ARRB2 is a regulatory protein involved in heterotrimeric G protein-coupled receptor desensitization and is known to regulate beta2-adrenergic receptor (beta2AR) function by enhancing beta2AR mediated nuclear translocation of ERK. ARRB2 binds to phosphorylated beta2ARs, thereby causing a significant impairment of their capacity to activate G(S) proteins. Along with AIP4, ARRB2 acts as an endosomal sorting molecule that mediates CXCR4 entry into a degradative pathway. It may also be involved in hormone-specific desensitization of TSH receptors. ARRB2 is a Ca2+ binding protein of the retinal outer segments and binds to P-rhodopsin and is also involved in synaptic transmission in photoreceptor cells. It is widely expressed in most of the tissues, especially neuronal tissues and spleen.