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Applied Biosystems™ BigDye™ Direct Cycle Sequencing Kit

The BigDye™ Direct Cycle Sequencing Kit, part of the BigDye™ product family, is a complete kit for your PCR and Sequencing needs.

Brand: Applied Biosystems™ 4458687

285.34 EUR valid until 2024-12-14
Use promo code "21632" to get your promotional price.

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Product Code. 15889856

428.00 EUR / 100 reactions

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Alert:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price

Includes: This kit contains BigDye™ Direct PCR Master Mix, Sequencing Master Mix, M13 Fwd Primer, M13 Rev Primer, and Control DNA CEPH 1347-02, This kit contains sufficient reagents for 24 reactions

This item is not returnable. View return policy

Description

A streamlined, simple procedure
Performance of all sequencing steps in a single reaction tube
Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
Substantial time savings versus previous workflows

Order Info

1 Kit

Specifications

Content And Storage	This kit contains BigDye™ Direct PCR Master Mix, BigDye™ Direct Sequencing Master Mix, BigDye™ Direct M13 Fwd Primer, BigDye™ Direct M13 Rev Primer, and Control DNA CEPH 1347-02. Store all components at -15 to -25°C and protect from light. PCR Master Mix should be stored at 4°C after first use. This kit contains sufficient reagents for 100 reactions.
Format	8-well Strip, 96-well Plate
For Use With (Application)	Comparative Sequencing (Germline Mutations - 30:70 Heterozygotes), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), GT-Rich or Difficult Template Sequencing, Standard Sequencing, Gap Closure, Homopolymer A or T, AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations -10:90 Heterozygotes)
Product Line	BigDye™
Shipping Condition	Dry Ice
Template Compatibility	BAC DNA, Cosmids, Lambda DNA, PCR Amplicons (10:90 Heterozygotes), Plasmid DNA (≤15Kb), Genomic DNA (Bacterial), Fosmids, PCR Amplicons (50:50 Heterozygotes), PCR Amplicons (30:70 Heterozygotes)

Description	BigDye™ Direct Cycle Sequencing Kit, 100 rxns, 8-well Strips, 96-well Plate, Fluorescent Dye Terminator Sequencing Technique, 3, Use with 130xl Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, 3730xl DNA Analyzer, GeneAmp™ 9700, Veriti™ Thermal Cycler
For Use With (Equipment)	3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, SeqStudio Genetic Analyzer, Veriti Thermal Cycler, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer
No. of Reactions	100
Product Type	Direct Cycle Sequencing Kit
Technique	Fluorescent Dye Terminator Sequencing

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For Research Use Only. Not for use in diagnostic procedures.