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Description
CCF4-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF4 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF4-AM into its negatively charged form, CCF4, which is retained in the cytosol. CCF4 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer™ technology.
Specifications
| Cell-Permeant | |
| Fluorescence | |
| LiveBLAzer™ | |
| CCF4-AM | |
| Beta-Lactamase Substrate | |
| Room Temperature |
| Store in freezer (-5 to -30°C). | |
| Solid | |
| 20mg | |
| Chemical Substrate | |
| Flow Cytometer, Microplate Reader |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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