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Gibco™ CHO-S-SFM II (without hypoxanthine or thymidine)
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CHO-S-SFM II (without hypoxanthine or thymidine)

Brand:  Gibco™ 31033020

Product Code. 11574416

  • 109.00 EUR / 500mL

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Description

Description

CHO-S-SFM II, is a complete, serum-free, low-protein (less than 100μg/ml) medium optimized for the growth and maintenance of Chinese hamster ovary (CHO) cells in suspension culture for production of recombinant proteins. This medium contains no thymidine or hypoxanthine (the supplemented medium is also available). This ready-to-use medium is suitable for the suspension culturing of CHO and other cells in batch, continuous, and perfusion culture systems. A CHO-S-SFM II medium Drug Master File (DMF) has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.

This medium contains components of animal origin. If growth factors or hydrolysates, found in competitor media, are to be avoided, consider CD CHO, CD OptiCHO™ or CD FortiCHO™ media.

When to use CHO-S-SFM II Medium
  • Your cells have been successfully cultured in CHO-S-SFM II medium in the past and your process is nearing a regulatory filing (DMF available)

    When to Consider Another Gibco™ Medium

    Consider CD FortiCHO™ medium when:
  • The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
  • Maximum batch culture cell densities and protein titers are needed
  • You have time to adapt cells into a new medium in an effort to maximize titers
  • Cell health at harvest is a priority for downstream processing

    Consider CD OptiCHO™ medium when:
  • You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
  • Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
  • Your CHO cell line is finicky, i.e. hard to grow or adapt

    Consider CD CHO medium when:
  • Your GS CHO cells are recommended to be grown in CD CHO medium
  • Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

    Consider CD DG44 medium when:
  • You use parental DG44 or DXB11 CHO cells (dhfr-)

    Design Your Own Media Specific to Your Needs
    Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco™ cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.
    TRUSTED_SUSTAINABILITY
    • Specifications

    Specifications

    Store in refrigerator (2–8°C) and protect from light.
    Low Protein, Serum-free
    Suspension Cell Culture
    Serum Free Medium (SFM)
    Glutamine, Sodium Pyruvate, Sodium Bicarbonate
    ≤0.25 EU/mL, Low
    Sustainable packaging
    Gibco™
    Room Temperature
    CHO
    1 X
    Liquid
    Serum-free
    No Phenol Red, No Thymidine, No Hypoxanthine
    Bottle(s)
    No antibiotics
    0.5 L
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    For Research Use Only. Not for use in diagnostic procedures.