Thermo Scientific™ DreamTaq™ Hot Start Green DNA Polymerase

Description

Thermo Scientific™ DreamTaq™ Hot Start Green DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. The enzyme is supplied with an optimized 10X DreamTaq Green buffer that includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel. The buffer contains magnesium chloride, which eliminates the need for extensive optimization of reaction conditions. DreamTaq Hot Start DNA Polymerase is also available without pre-added density reagent and electrophoresis tracking dyes, as well as in master mix format.

DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.

DreamTaq Hot Start Green DNA Polymerase is formulated to enhance productivity through:

• Better reaction outcomes
  • Higher yield of target amplicons from low template amounts
  • Increased reaction specificity due to reliable hot-start technology
  • Wider range of amplicon lengths—routine amplification of genomic DNA fragments up to 6 kb
• Enhanced convenience
  • Room temperature reaction set-up
  • Minimized optimization of Mg²⁺ concentration and of primer annealing temperatures due to optimized reaction buffer
  • Direct loading of PCR products on a gel with DreamTaq Green buffer

Applications

• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning