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Gibco™ ExpiCHO™ Expression System Kit
The ExpiCHO Expression System Kit combines the power of rapid, ultra-high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells.
Brand: Gibco™ A29133
2316.78 EUR valid until 2024-12-14
Use promo code "21632" to get your promotional price.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price
Includes: 2 vials frozen ExpiCHO-S™ cells, 1 bottle 1000mL ExpiCHO™ Expression Medium, 1 ExpiFectamine™ CHO transfection kit for 1 L of culture, 1 vial Antibody Expressing Positive Control Vector, 1 bottle 100mL OptiPRO™ SFM; store all components at +2° - +8° C, except frozen cells stored in liquid nitrogen
Description
The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium. Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed.
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
The ExpiCHO Expression System enables:
- Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
- Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
- Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Use of one-third to one-half less plasmid DNA than other transfection reagents
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
- Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.
ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.
All components of the system are animal origin–free.
Specifications
| Expression System | |
| Transfection | |
| • 2 vials frozen ExpiCHO-S cells • 1000 mL ExpiCHO Expression Medium • 1 ExpiFectamine CHO Transfection Kit for 1 L of culture • 1 vial Antibody Expressing Positive Control Vector • 100 mL OptiPRO SFM Store all components at 2-8°C, except frozen cells which should be stored in liquid nitrogen. |
|
| CHO-S, SFM Adapted | |
| ExpiCHO™ | |
| CMV |
| Untagged | |
| 1 kit | |
| Approved for shipment at Room Temperature or on Wet or Dry Ice | |
| Mammalian | |
| Protein Production, Constitutive Expression |