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Thermo Scientific™ DreamTaq™ Green Buffer (10X)
Thermo Scientific™ 10X DreamTaq™ Green Buffer is a proprietary PCR buffer that contains KCl and (NH4)2SO4 at a ratio optimized for robust performance of DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase in PCR.
Brand: Thermo Scientific™ B71
This item is not returnable.
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Description
Thermo Scientific™ 10X DreamTaq™ Green Buffer is a proprietary PCR buffer that contains KCl and (NH4)2SO4 at a ratio optimized for robust performance of DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase in PCR and includes MgCl2 at a concentration of 20 mM. In addition, the buffer includes a density reagent and two tracking dyes. The density reagent allows direct loading of PCR products on a gel. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress.
The dyes have excitation peaks at 424 nm and 615 nm. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq™ Buffer (10X) or purifying the PCR product using the GeneJET PCR Purification Kit prior to analysis.
Specifications
| Dream TAQ Green Buffer | |
| DreamTaq Hot Start DNA Polymerase, DreamTaq DNA Polymerase | |
| DreamTaq Green Buffer | |
| Wet or Dry Ice | |
| 10X |
| • 10X DreamTaq Green Buffer (with 20 mM MgCl2), 4 x 1.25 mL Store in a non-frost-free freezer at -20°C. |
|
| Nonidet P40 Ethyl phenolpoly(ethyleneglycolether) 9016-45-9 ( <0.9 ), Polyethylene glycol 25322-68-3 ( 7-13 ), Sucrose 57-50-1 ( 10-30 ) | |
| 4 x 1.25 mL | |
| Hot-start PCR |
For Research Use Only. Not for use in diagnostic procedures.