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Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Brand:  Thermo Scientific™ B49

54.58 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.



Product Code. 10399519

  • 95.75 EUR / 1 L

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Description

Description

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Usage Recommendations

  • Use fresh 1X TAE both for the gel and for the electrophoresis run
  • To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

  • The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

  • 40mM Tris
  • 20mM acetic acid
  • 1mM EDTA
  • pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

50X
1 L
TAE Buffer
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Safety and Handling

Safety and Handling

Product Identifier
  • TAE Buffer (Tris-acetate-EDTA) (50X)

Signal Word
  • Warning

Hazard Category
  • Specific target organ toxicity after repeated exposure Category 2

Hazard Statement
  • H373-May cause damage to organs.

Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P314-Get medical advice/attention if you feel unwell.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : acetic acid

SDS
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Special Offers

For Research Use Only. Not for use in diagnostic procedures.