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Thermo Scientific™ PaeI (SphI) (10 U/μL)

The PaeI (SphI) restriction enzyme recognizes GCATG^C sites and cuts best at 37°C in B buffer (Isoschizomers: BbuI, SphI).

99.75 EUR - 423.00 EUR

Specifications

Concentration 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme PaeI (SphI)
Compatible Buffer 10X Buffer B
Sensitive to Heat Inactivation Yes
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Products 2
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Product Code Brand Quantity Price Quantity & Availability  
10691041
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Thermo Scientific™
ER0601
500 U
99.75 EUR
500 units
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10170790
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Thermo Scientific™
ER0602
2500 U
423.00 EUR
2500 units
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Description

Description

Lambda DNA digested with PaeI (SphI), 0.7% agarose, 6 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

  • Superior quality -stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities
Specifications

Specifications

10 U/μL
PaeI (SphI)
Yes
No
Traditional Cloning
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer B
37°C
Restriction Enzyme
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For Research Use Only. Not for use in diagnostic procedures.