missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Cytiva First-Strand cDNA Synthesis Kit
Shop All Cytiva Products
Click to view available options
Quantity:
55 Reactions
Unit Size:
1 set
Description
Includes
Bulk first-strand cDNA reaction mixes (Cloned murine reverse transcriptase, RNase inhibitor, RNase/DNase-free BSA, dATP, dCTP, dGTP, and dTTP in aqueous buffer), mRNA standard, DTT solution, RNase-free water, pd(N)6 primer, and Not I-(dT)19 bifunctional primer
- Preassembled bulk reaction mixes save time and minimize potential for pipetting errors
- Primers are not included in the preassembled reaction mixes, allowing use of a primer of choice
- Two primers are included with the kit
- pd(N)6 primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail
- Not I-(dT)18 bifunctional primer can be used to prime selectively on mRNA having a poly(A) tail and can also be used as a PCR primer following first-strand synthesis
- The Not I restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products
Specifications
Specifications
| Content And Storage | -15°C to -30°C |
| Description | Designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. |
| For Use With (Application) | RT-PCR |
| Includes | Bulk first strand cDNA reaction mixes, mRNA standard, DTT solution, RNase-free Water, pd(N)6 primer and NotI-(dT)18 bifunctional primer |
| No. of Reactions | 55 |
| Product Type | First-Strand cDNA Synthesis Kit |
| Quantity | 55 Reactions |
Seguridad y manipulación
Nombre del producto
- First-Strand cDNA Synthesis Kit, 55 reactions
- EUH210-Safety data sheet available on request.
- UNKNOWN ACUTE-3 per cent of the mixture consists of component(s) of unknown acute toxicity.
- UNKNOWN AQUATIC-Contains 25.5% of components with unknown hazards to the aquatic environment.
Corrección del contenido de un producto
Proporcione sus comentarios sobre el contenido del producto rellenando el siguiente formulario.
Título del producto
¿Detecta una oportunidad de mejora?Comparta una corrección de contenido