Thermo Scientific™ Fraser Broth (Tube)

Description

Selectively enrich and isolate Listeria spp. from food and environmental samples with Thermo Scientific™ Oxoid™ Fraser Broth in ready-prepared tubes. The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.

Remove the need for pipetting, dispensing or autoclaving with convenient, pre-filled and ready-to-use tubes of Fraser Broth.

• Convenient: pre-filled tubes are ready for immediate use.
• Simple: easy-to-use with minimum training.
• Selective: medium is selective for Listeria.

The medium contains ferric ammonium citrate and lithium chloride. All Listeria spp. hydrolyse aesculin to aesculetin. Aesculetin reacts with ferric ions which results in blackening. Another possible advantage to the addition of ferric ammonium citrate is that it has been shown that ferric ions enhance the growth of Listeria monocytogenes¹. Lithium chloride is included in the medium to inhibit the growth of enterococci which can also hydrolyse aesculin. Blackening of the medium is presumptive evidence of the presence of Listeria. Contrary to early indications, cultures which do not blacken cannot be assumed to be Listeria-free. Therefore, all Fraser Broth enrichment cultures should be sub-cultured to plating medium.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific™ br