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Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System
Rapid, ultrasensitive detection of β-galactosidase in reporter gene assays. Thermo Scientific Galacto-Light Plus β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.
Brand: Invitrogen™ T1007
Includes: Lysis solution, substrate, reaction buffer and accelerator
Description
- Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
- Assay sensitivity is 100- to 1000-fold better than with either isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
- Highly sensitive assay with wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Ready-to-Use
The Galacto-Light Plus system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.
Specifications
- Assay Type: β-Gal (lacZ) Assay
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: Galacton-Plus
- Substrate Properties: Chemical Substrate
- Substrate Type: Beta-Gal Substrate
- Target Enzyme: Beta-Galactosidase
Specifications
| β-Galactosidase Reporter Gene Assay System | |
| Luminometer (Microplate) | |
| This kit contains the lysis solution, substrate, reaction buffer, and accelerator. Store at 4°C. |
|
| High-throughput Compatible | |
| End Point | |
| Galacton-Plus™ | |
| Chemical Substrate | |
| Galacto-Light Plus™, NovaBright™ | |
| 200 Assays |
| Chemiluminescence | |
| β-Gal (lacZ) Assay | |
| Mammalian Cells | |
| Unconjugated | |
| Cell Cultures | |
| Beta-Gal Substrate | |
| Beta-Galactosidase | |
| Enzyme Assay | |
| Liquid |
For Research Use Only. Not for use in diagnostic procedures.