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Thermo Scientific™ GeneBLAzer™ GR-UAS-bla HEK 293T Cells

Brand: Thermo Scientific™ K1687

Product Code. 10083913

10380.00 EUR / Each

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Estimated Shipment: 20-01-2025
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Description

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-blaHEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-GR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. GR-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of Dexamethasone. In addition, GR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

Alternate Agonists	Tetrac, T4
Assay Entry	Cell-based beta lactamase reporter gene
Content And Storage	GR-UAS-bla HEK 293T cells (K1687) Shipping: Dry Ice Storage: -80°C (liquid nitrogen), Liquid Nitrogen
Description	GeneBLAzer™ GR-UAS-bla HEK 293T Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, 1-850 Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
Species	Human
Includes	GeneBLAzer™ GR-UAS-bla HEK 293T cells - Includes: GR-UAS-bla HEK 293T cells, Protocol, Certificate of Analysis
Primary Agonist	T3 Hormone
Quantity	2 x 106 Cells, 2 x 10⁶ cells
Sample Type	293T (HEK)
Target Entry	NR3C1, GR

Antagonists	1-850
Cell State	Dividing Cells
Detection Method	Fluorescence, Fluorescent
For Use With (Application)	Pharma and biopharma, target and lead identification and validation
Druggable Target	Nuclear Receptors
No. of Cells	2 x 10⁶ Cells
Product Line	GeneBLAzer™
Product Type	HEK 293T Cells
Reporter Gene	BLA (Beta-Lactamase)
Type	Cell Line

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Thermo Scientific™ GeneBLAzer™ GR-UAS-bla HEK 293T Cells

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