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Invitrogen™ GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit

GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit

Brand:  Invitrogen™ K1619

Product Code. 10791614

  • 966.00 EUR / Each
Estimated Shipment: 20-01-2025
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Description

Description

The GeneBLAzer™ PAC1-CRE-bla CHO-K1 cells contain the human Adenylate Cyclase Activating Polypeptide 1 Receptor 1 (ADCYAP1R⁄PAC1) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE). The PAC1-CRE-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, PAC1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

NM_001118
Division-Arrested Cells
Fluorescence
Single Cell Clone Origin
8 x 106 cells
ADCYAP1R1, PAC1
CHO-K1
GPCR
Cell-based beta lactamase reporter gene
K1619 GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ PAC1 CHO-K1 DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70μg
• Solution D
• GeneBLAzer™ PAC1 CHO-K1 DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ PAC1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
ADCYAP1R1
GeneBLAzer™
BLA (Beta-Lactamase)
Division-Arrested Cells Assay Kit
GeneBLAzer™
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