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Thermo Scientific™ GeneBLAzer™ RXR beta DA Assay Kit
GeneBLAzer™RXR beta DA (Division Arrested) cells and RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Brand: Thermo Scientific™ K1405
This item is not returnable.
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Description
GeneBLAzer™RXR beta DA (Division Arrested) cells and RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR beta DA cells and RXR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of 9-cis retinoic acid (9-cis-RA). In addition, RXR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
Specifications
| Progesterone | |
| Cell-based beta lactamase reporter gene | |
| Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate: • RXR beta HEK 293T DA cells (K1405A) • LiveBLAzer™-FRET B/G Loading Kit, 70 μg Shipping: Dry Ice Storage: -80°C (liquid nitrogen) |
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| Fluorescence | |
| RXRβ | |
| R5020 | |
| 1 Plate | |
| 293T (HEK) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
| RU 486 | |
| Division-Arrested Cells | |
| GeneBLAzer™ RXR beta DA Assay Kit, 1Plate, ∽ 4,800,000 cells/vial Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Adherent Growth Properties, –80°C/Liquid Nitrogen Storage | |
| Cellular imaging, pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Cell analysis, cell-based reporter assays, cell-based and lactamase assays, drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based ADME/tox assays, cellular adme assays, nuclear receptor cellular adme assays | |
| RXR beta HEK 293T DA cells (K1405A) LiveBLAzer™-FRET B/G Loading Kit, 70μg | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| RXR beta, NR2B2, RXRB | |
| 293T (HEK) | |
| Nuclear Receptors |
For Research Use Only. Not for use in diagnostic procedures.