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Gibco™ DMEM/F-12
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DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

34.72 EUR

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Specifications

Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Cell Line MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
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Product Code Brand Quantity Price Quantity & Availability  
11554546
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Gibco™
11320033 		500 mL
34.72 EUR
500mL
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Description

Description

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using DMEM/F-12

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
1 X
DMEM (Dulbecco's Modified Eagle Medium)/F-12
Sterile-filtered
cGMP-compliant under the ISO 13485 standard
Room Temperature
MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Animal Origin-free
Liquid
Standard Serum Supplementation
High Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Gibco™
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Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 12 Months From Date of Manufacture

SDS
Documents

Documents

Product Certifications

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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