missing translation for 'onlineSavingsMsg'
Learn More

Gibco™ Ham's F-12 Nutrient Mix, powder

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

35.74 EUR - 215.00 EUR

Specifications

Content And Storage Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Type Primary Rat Astrocytes
Form Powder
Product Type Ham's F-12 Nutrient Mixture
With Additives Glutamine, Phenol Red, Sodium Pyruvate
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15480604
View Documents
Gibco™
21700075
10 x 1 L
35.74 EUR
Pack of 10
Please to purchase this item. Need a web account? Register with us today!
10472882
View Documents
Gibco™
21700018
50 L
215.00 EUR
Each
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With: L-glutamine, Phenol Red
Without: Sodium Bicarbonate

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Specifications

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Powder
Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
Room Temperature
Primary Rat Astrocytes
Ham's F-12 Nutrient Mixture
No HEPES, No Sodium Bicarbonate
Gibco™
Videos
Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 36 Months From Date of Manufacture

SDS
Documents

Documents

Product Certifications

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.