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  7. Gibco™ Ham's F-12K (Kaighn's) Medium
  8. 10667440

Gibco™ Ham's F-12K (Kaighn's) Medium
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Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

Brand:  Gibco™ 21127030

283.21 EUR valid until 2024-12-31
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Product Code. 10667440

  • 359.00 EUR / Pack of 10

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Description

Description

Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:

With: L-glutamine, Phenol Red

Without: HEPES

The complete formulation is available.

Using Ham's F-12K

Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

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Specifications

Specifications

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Animal Origin-free
Liquid
Sterile-filtered
Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
cGMP-compliant under the ISO 13485 standard
10 x 500 mL
Human Hepatocytes
1 X
Ham's F-12K (Kaighn's) Medium
Sterile-filtered
No HEPES
Gibco™
Room Temperature
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