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  8. 16271891

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Brand:  Applied Biosystems™ 4388950

1893.50 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

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Product Code. 16271891

  • 2705.00 EUR / 500 reactions

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Includes: 1 x 500 uL enzyme mix (MuLV and RNase inhibitor protein) (20X), 5 x 1 mL RT Buffer Mix (2X) (includes dNTPs, random octamers, and oligo dT-16)
This item is not returnable. View return policy

Description

Description

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts.

Features of the High Capacity RNA-to-cDNA Kit include:
• Fast reaction time (typically 30–60 min)
• Convenient and simple workflow with few pipetting steps
• Reliable reverse transcription of both abundant and limited targets
• Optimized two-step protocol enables multiple PCR reactions from a single RT reaction

Streamline cDNA synthesis while maintaining detection sensitivity
Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude. This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes. The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 20X Enzyme mix (1 x 500 μL)
• 2X RT Buffer Mix (5 x 1 mL)

Store at –15 to –25°C.
2X RT Buffer Mix can be stored at 2–8°C for up to 6 months.
30 to 60 min.
37°C
MultiScribe
Dry Ice
First-Strand cDNA
Premixed Components
Up to 7 kb
Kit
Reverse Transcription
500 reactions
Yes
Real Time PCR (qPCR), RT-PCR
500 Reactions
Reverse Transcription
RNA
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For Research Use Only. Not for use in diagnostic procedures.