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Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns
Used for method optimization and parameter screening
Brand: Cytiva 28-9782-44
This item is not returnable.
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Description
- Based on 6% highly cross-linked agarose with an average bead size of 90μm
- Processed flow properties and high loading capacities
- Bed height of 10cm is designed to allow method optimization and parameter screening
- Easily connected in series to achieve 20cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
- Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity
- Bed volume: 4.7mL
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 90μm
- Flow velocity: <600cm/hr or <450cm/hr
- Material (Column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
Blue Sepharose
- Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
- Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
- Prepacked with Nickel Sepharose 6 Fast Flow
- Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
- Prepacked with DEAE Sepharose Fast Flow
- Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium
Specifications
| 0.1M HCl, 0.1M NaOH, 8M Urea | |
| 4.7 mL | |
| 7.7 mm | |
| 3.93 in. | |
| <450 cm/hr. | |
| Ni Sepharose | |
| 3 to 12 | |
| 6% Cross-linked Agarose | |
| Prepacked Fast Flow Column |
| HiScreen Ni Sepharose 6 FF column | |
| 0.3 in. | |
| 1 x 4.7mL | |
| 100mm | |
| Polypropylene | |
| 1.5 bar | |
| 45 to 165μm | |
| 4° to 30°C | |
| Purification of His-tagged Proteins with immobilized Metal ion affinity chromato graphy |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol, nickel
- UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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