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Invitrogen™ Interferon alpha (Cell culture) Human ELISA Kit
Measure levels of human interferon alpha (IFNa) in human serum and human plasma samples using 96-well plates.
Brand: Invitrogen™ 411102
This item is not returnable.
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Description
Assay IFN-a (human interferon alpha) levels in human serum, plasma and tissue culture media with these Thermo Scientific™ Human IFN alpha ELISA Kits (for Serum Samples).
IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related,species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans,the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities),and single beta,omega,epsilon and kappa isoforms. Homologous molecules are found in many species,including rats and mice (and most mammals),and have been identified in birds,reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells,is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.
Specifications
| 12.5-1000pg/mL | |
| HRP | |
| ELISA Kit | |
| No significant cross-reaction with human IFN-ß, IFN-g or IFN-w; mouse or rat IFN-a, IFN-ß, or IFN-g; bovine IFN-t | |
| Human | |
| IFNA1,IFNA2 | |
| <8% | |
| HRP | |
| Intra-assay CV ≤ 8%; inter-assay CV ≤ 8% | |
| RUO | |
| 2°C to 8°C | |
| 1 hr. 20 min. |
| Human IFN alpha ELISA Kit (Serum Samples) | |
| Colorimetric ELISA (TMB substrate) with Plates | |
| Supernatant | |
| 5 x 96 microplate wells | |
| Colorimetric Microplate Reader | |
| <8% | |
| Plate Sealers, 20 ea, Concentrate Diluent, 60mL, Sample Diluent, 150mL, Antibody Concentrate, 1 vial, Dilution Buffer, 150mL, HRP Conjugate Concentrate, 1 vial, Human IFN Alpha Standard 10,000pg/mL, 2.5mL, Precoated 96-well Plates, 5 ea, Stop Solution, 60mL, Wash Solution Concentrate, 250mL, TMB Substrate Solution, 60mL | |
| Absorbance at 450nm | |
| 5 x 96 Tests | |
| Supernatant, 100 μL | |
| Human | |
| 3 hr. 15 min. |