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Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.

412.00 EUR - 1914.00 EUR

Specifications

GC-Rich PCR Performance High
Polymerase AccuPrime GC-Rich DNA Polymerase
Reaction Speed Standard
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR
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Includes: AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).

Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10515013
View Documents Promotion Details
Invitrogen™
12337016
200 Reactions
412.00 EUR
Each
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10033322
View Documents Promotion Details
Invitrogen™
12337024
1000 Reactions
1914.00 EUR
Each
Please to purchase this item. Need a web account? Register with us today!
Description

Description

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

AccuPrime GC-Rich DNA Polymerase features

  • High yields for targets up to 5 kb in length
  • AccuPrime accessory proteins for improved PCR specificity
  • Sensitivity down to 5 ng of template DNA

Robust and specific amplifications

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition

One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

High
Standard
Hot-start PCR
Built-In Hot Start
Separate Components
AccuPrime GC-Rich DNA Polymerase
Wet or Dry Ice
2X
Blunt
5 kb or less
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.