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Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
412.00 EUR - 1914.00 EUR
Specifications
GC-Rich PCR Performance | High |
---|---|
Polymerase | AccuPrime GC-Rich DNA Polymerase |
Reaction Speed | Standard |
Shipping Condition | Wet or Dry Ice |
For Use With (Application) | Hot-start PCR |
Includes: AccuPrime GC-Rich DNA Polymerase (100μL, at 2U/μL) is supplied with two 5X reaction buffers and a separate vial (1mL) of 50mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime accessory proteins. Reaction buffer A (1mL) is for genomic targets. Reaction buffer B (1mL) is for use with cDNA, plasmid, or lambda targets. Sufficient reagents are provided for 200 amplification reactions of 25μL each (at 1 unit of enzyme per reaction).
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
10515013
|
Invitrogen™
12337016 |
200 Reactions |
412.00 EUR
Each |
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10033322
|
Invitrogen™
12337024 |
1000 Reactions |
1914.00 EUR
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).
AccuPrime GC-Rich DNA Polymerase features
- High yields for targets up to 5 kb in length
- AccuPrime accessory proteins for improved PCR specificity
- Sensitivity down to 5 ng of template DNA
Robust and specific amplifications
The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
Unit definition
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min. at 74°C.
Order Info
Approved for shipment on Wet or Dry Ice
Specifications
High | |
Standard | |
Hot-start PCR | |
Built-In Hot Start | |
Separate Components |
AccuPrime GC-Rich DNA Polymerase | |
Wet or Dry Ice | |
2X | |
Blunt | |
5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.