Invitrogen™ BaculoDirect™ N-Term Transfection Kit

Description

• Engineered to include attR sites for quick and efficient recombination with Gateway entry clone
• Gene of interest is recombined from entry clone into BaculoDirect Linear DNA using a simple, one-hour LR reaction
• Resulting reaction mix contains recombinant baculovirus carrying gene of interest and is used directly to transfect insect cells
• Need for transforming bacteria and isolating large bacmid or co-transfection of a transfer vector and linear baculovirus DNA into insect cells is eliminated for reduced hands-on time
• Purified baculovirus can be isolated in less than one week
• N-terminal V5-His tag for detection with Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
• TEV protease cleavage site for removal of V5-His tag following purification (BaculoDirectN-Term Linear DNA)
• C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)
• Expression can be detected as early as first baculovirus infection

• Gateway Linear DNA: Designed for rapid cloning with a Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells
• Strong polyhedrin promoter for high-level expression
• R sites for efficient recombination with any attL-flanked Gateway entry vector
• TK gene for negative selection using ganciclovir
• N-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
• TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect N-Term Linear DNA)
• C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)

Baculovirus Production and Titering/Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis