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Invitrogen™ Champion™ pET200 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein in E. coli during expression
Brand: Invitrogen™ K20001
1046.76 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: 200ng of linearized, topoisomerase I-activated Champion pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. The TOP10 box contains 21 x 50μL aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. The BL21 Star(DE3) box contains 21 x 50μL aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid.
Description
- Based on T7 expression vectors originally developed by Studier and colleagues, high-level expression is achieved because the T7 RNA polymerase is more processive than native E. coliRNA polymerase and is dedicated to transcription of gene of interest
- Protein production is further enhanced in system by expression strain BL21 StarE. coli, which significantly improves stability of mRNA transcripts and increases protein expression up to ten-fold
- Simplified, efficient Directional TOPO cloning allows to quickly enter a ChampionpET Expression vector
- These kits feature linearized, topoisomerase I-activated ChampionpET expression vectors for five-minute directional cloning to facilitate gene expression with proofreading enzyme used for PCR, resulting in fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for gene expression, reducing the time spent on colony screening
Bacterial ExpressionProtein ExpressionProteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| T7, lacO | |
| None | |
| Kanamycin (KanR) | |
| EK (Enterokinase) Recognition Site | |
| Cell-Based Expression | |
| Directional TOPO | |
| pET |
| IPTG | |
| TOPO Expression Kit | |
| Each Champion™ pET Directional TOPO™ Expression Kit is provided as a complete expression system. The Directional TOPO™ Expression box contains 200 ng of linearized, topoisomerase I-activated Champion™ pET vector; sterile water; dNTPs; 10X PCR Buffer; salt solution; control template and primers; primers for sequencing or PCR screening; and an expression control. Store at -20°C. The One Shot™ TOP10 box contains twenty-one 50-μl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot™ BL21 Star™(DE3) box contains twenty-one 50-μl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. Kits with Lumio™ Technology include 20 μl of the Lumio™ Detection Reagent. Store at -20°C. Guaranteed stable for 6 months when properly stored. | |
| BL21 Star™(DE3) | |
| E. coli | |
| His Tag (6x), Xpress Epitope Tag | |
| 20 reactions | |
| One Shot™ |
For Research Use Only. Not for use in diagnostic procedures.