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Invitrogen™ pcDNA™3.1 Directional TOPO™ Expression Kit
Linearized, topoisomerase I-activated pcDNA3.1D/V5-His-TOPO vector for 5-minute directional cloning and subsequent high-level expression
Brand: Invitrogen™ K490001
892.32 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: Box 1: 20μL pcDNA 3.1D/V5-His-TOPO (15–20ng/μL), 10μL dNTP Mix, 50μL Salt Solution, 1mL Sterile Water, 20μL T7 Sequencing Primer (0.1μg/μL), 20μL BGH Reverse Sequencing Primer (0.1μg/μL), 10μL Control PCR Template (0.1μg/μL), 10μL Control PCR Primers (0.1μg/μL each, 10μL Expression Plasmid (0.5μg/μL); Box 2: 6mL S.O.C. Medium, 21 × 50μL TOP10 Cell, 50μL pUC19 Control DNA
Description
- Proofreading enzyme is used: fewer errors in cloned genes
- Greater than 90% of clones are in correct orientation for expression, reducing time spent screening colonies for clone orientation
- CMV promoter: high expression levels
- C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody
- 6xHis tag for rapid protein purification on nickel-chelating resin
Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Dry Ice
Specifications
| Constitutive | |
| CMV | |
| Geneticin™ (G-418) | |
| His Tag (6x), V5 Epitope Tag | |
| 20 Reactions | |
| TOPO™, pcDNA™ |
| Transfection | |
| TOPO Expression Kit | |
| Box 1 (Store at -20°C): • 20 μl pcDNA™3.1D/V5-His-TOPO™ (15–20 ng/μl) • 10 μl dNTP Mix • 50 μl Salt Solution • 1 ml Sterile Water • 20 μl T7 Sequencing Primer (0.1 μg/μl) • 20 μl BGH Reverse Sequencing Primer (0.1 μg/μl) • 10 μl Control PCR Template (0.1 μg/μl) • 10 μl Control PCR Primers (0.1 μg/μl each) • 10 μl Expression Plasmid (0.5 μg/μl) Box 2 (Store at -80°C): • 6 ml S.O.C. Medium (may be stored at 4°C or room temperature) • 21 × 50 μl TOP10 Cells • 50 μl pUC19 Control DNA |
|
| Directional TOPO | |
| Directional TOPO Vectors, pcDNA | |
| Constitutive Expression |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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