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Invitrogen™ SuperScript™ One-Cycle cDNA Kit for use with Thermo Scientific™ One-Cycle Assays
Conveniently contains all of the reagents required for double-stranded cDNA synthesis within the GeneChip™ workflow
Brand: Invitrogen™ A10752030
Includes: 50μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10mM dNTP, SuperScript™ II (200u/μL), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water
Description
The kit includes Invitrogen's proprietary RNase H reduced activity SuperScript™ II Reverse Transcriptase and a qualified oligo (dt)-containing primer to perform reactions standard to Affymetrix– protocol. Designed to consistently produce higher yields of full-length cDNA for Affymetrix One-Cycle assays using either total RNA or poly A+ selected RNA (mRNA).
Order Info
Shipping Condition: Dry Ice
Specifications
| 50 μM T7-Oligo(dT) Primer, 5X 1st Strand Reaction Mix, 0.1M DTT, 10 mM dNTP, SuperScript™ II (200u⁄ul), 5X 2nd Strand Reaction Mix, E.Coli DNA Ligase, E.Coli DNA Polymerase I, RNase H, T4 DNA Polymerase, 0.5M EDTA, RNase-free Water All components are guaranteed stable for 6 months when properly stored. |
|
| Kit | |
| cDNA (Double-stranded) | |
| cDNA | |
| No | |
| Direct Labeling | |
| cDNA Kit | |
| Dry Ice |
| Biotin-based | |
| Biotin | |
| Poly(A+) RNA, Total RNA | |
| For generating double-stranded cDNA | |
| Affymetrix GeneChip™ | |
| SuperScript™ | |
| 30 Reactions |
For Research Use Only. Not for use in diagnostic procedures.