Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.

• Fast and convenient: 15-minute, room-temperature ligation
• Efficient: blue/white screening and >80% clones with correct insert
• Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
• Hassle-free: eliminates any enzymatic modifications of the PCR product
• Streamlined: does not require the use of PCR primers that contain restriction sites

• Provides 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 promoter for in vitro RNA transcription and sequencing
• Versatile polylinker with flanking EcoR I sites for easy excision of insert
• M13 forward and reverse primer sites for sequencing
• How TA Cloning Works
• Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
• Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
• Allows PCR inserts to ligate efficiently with vector

Cloning, PCR Cloning