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Cytiva Lentil Lectin Sepharose™ 4B Medium

Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules

Brand: Cytiva 17-0444-01

Product Code. 11544905

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Description

Lentil lectin coupled to Sepharose 4B via cyanogen bromide activation
Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
Retains binding in 1% deoxycholate; especially suited for purification of detergent-solubilized membrane proteins
Ligand concentration 2 mg Lentil lectin/ml; binding capacity for porcine thyroglobulin approx. 16mg/mL medium
Average particle size 90μm
Stable in the pH range 3-10, and in the presence of all commonly used buffers (Chelating agents and 8M urea to be avoided)
Has more equal affinity for α-D-mannose and α-D-glucose compared to Con A

Supplied in 20% ethanol containing 0.9% NaCl, 1mM Ca²⁺ and 1mM Mn²⁺

Specifications

Ligand Concentration	2mg Lentil lectin/mL Medium
Particle Size	90 μm
Quantity	25 mL
For Use With (Application)	Used for purification of a number of Glycoproteins and other Carbohydrate containing molecules

Product Suggestions Safety and Handling

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P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
P280-Wear protective gloves/protective clothing/eye protection/face protection.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

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