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Gibco™ Advanced DMEM/F-12
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Brand: Gibco™ 12634028
315.20 EUR valid until 2024-12-20
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Description
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES
The complete formulation is available.
Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Animal Origin | |
| Liquid | |
| Reduced Serum | |
| High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA) | |
| Sustainable packaging | |
| Gibco™ | |
| Room Temperature |
| MRC-5, SP2, Vero, WI-38 and Jurkat | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
| Sterile-filtered | |
| No Glutamine, No HEPES | |
| cGMP-compliant under the ISO 13485 standard | |
| 10 x 500 mL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Methyl benzoate
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
Hazard Statement
- H302-Harmful if swallowed.
Precautionary Statement
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
Supplemental information
- MIXTURE LIST-Contains : Methyl benzoate
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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