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  5. IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion

IBA Lifesciences pCSG-IBA Mammalian Expression Vector with No Secretion
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Product Code. 15623878 Shop All IBA Lifesciences Products
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Vector:
pCSG-IBA103
pCSG-IBA105
pCSG-IBA123
pCSG-IBA143
pCSG-IBA145
pCSG-IBA162
pCSG-IBA164
pCSG-IBA167
pCSG-IBA168
pCSG-IBA23
pCSG-IBA3
pCSG-IBA43
pCSG-IBA45
pCSG-IBA5
pCSG-IBA62
pCSG-IBA64
pCSG-IBAwt1
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5µg
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Código de producto. 15623878

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Descripción

Designed for high-level episomal expression in mammalian hosts under G418 selection. IBA Lifesciences GmbH Mammalian Expression Vector with No Secretion is a large expression vector with universal features for transient expression as well as for generation of stable cell lines (NeoR).

pCSG-IBA mammalian expression vectors

  • Designed for high-level episomal expression in mammalian hosts under G418 selection containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7)
  • Stable cell lines can be selected by the neomycin resistance gene (NeoR).
  • In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells.

Please note that cloning into pCSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry-vector is possible.

Especificaciones

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Species Mammalian
Protein Tag Twin-Strep-tag, FLAG-tag
Cloning Method Direct Cloning
Concentration 250 ng/μL
Primer Forward
Quantity 5 μg
Vector pCSG-IBA162
For Use With (Application) Episomal expression (oriP, EBNA-1)
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