Invitrogen™ PED-A1 (N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine) (Phospholipase A1 Selective Substrate)

Description

Molecular Probes phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells.

• Label (Ex/Em): PED-A1
• Specificity: PLA₁
• Cleavage product: BODIPY™ FL C5
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry
• Cell-Permeant

Note: Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Enzyme and Protein Activity Assays, Lipase and Phopholipase Activity, Proteins, Expression, Isolation and Analysis

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