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Invitrogen™ NA-XTD™ Influenza Neuraminidase Assay Kit
Next-generation chemiluminescence-based assay for long-lived emission kinetics for quantitation of influenza neuraminidase inhibitor resistance. Thermo Scientific NA-XTD Influenza Neuraminidase Assay Kit can be used for virus quantitation in media samples
Brand: Invitrogen™ 4457535
Includes: Influenza Neuraminidase Assay Kit includes 1000X NA-XTD Substrate, NA Sample Prep Buffer, NA-XTD Assay Buffer, NA-XTD Accelerator and 10 detection microplates Influenza Neuraminidase Assay Reagent Set includes 1000X NA-XTD Substrate, NA Sample Prep Buffer
Description
The NA-XTD kit includes detection reagents and microplates, eliminating the need for luminometers equipped with a reagent injector, and improving ease-of-use. This kit also includes complete assay protocols for quantitating sensitivity to neuraminidase inhibitors in various influenza virus isolates including: human influenza types A and B, A/H1N1 pandemic, avian, equine and porcine viruses.
- Superior performance - improved, long-lived light emission kinetics and read-time flexibility
- High sensitivity - high assay signal-to-noise, detection of low-virus concentrations, and wide assay dynamic range
- Simple instrumentation requirement - no reagent injectors or luminometer needed
- Multiple applications - neuraminidase inhibitor IC50 assays and cell-based virus quantitation
Long-Lived Light Emission Kinetics and Read-Time Flexibility
The NA-XTD assay yields much longer-lived light emission kinetics than does the NA-Star assay, eliminating the need for luminometer instrumentation with reagent injectors and enabling read-time flexibility and batch-mode processing of assay plates. NA-XTD assay signal half-life is approximately 2 hours, longer than the 5-10 minute half-life of the NA-Star assay signal. IC-50 values determined from data collected immediately or up to 3 hours following addition of NA-XTD Accelerator solution are identical.
High Sensitivity
The NA-XTD chemiluminescent assay provides higher detection sensitivity (better low-end detection limit), higher assay signal-to-noise ratio, and wider assay dynamic range than fluorescent assays with the MUNANA substrate. The NA-XTD assay also typically demonstrates slightly higher signal-to-noise than the NA-Star assay. The NA-XTD assay provides 2 to 50-fold higher sensitivity by signal-to-noise ratio than MUNANA-based fluorescence assays, depending on the virus isolate. The NA-XTD assay provides a dynamic range of detection of 3 to 4 orders of magnitude of neuraminidase enzyme concentration, compared to 2 to 3 orders of magnitude range with fluorescent MUNANA assays. The wide chemiluminescent neuraminidase assay range enables determination of IC-50 values over a wide range of virus concentrations, eliminating the need to titer virus prior to performing IC50 determination assays.
Simple Instrumentation Requirement
Optimally, a small sample of culture media is removed and assayed directly with NA-XTD reagents, permitting multiple samples to be assayed over time.
Specifications
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- High Throughput Compatibility: High Throughput-Compatible
- Readout: End Point
- Substrate: NA-XTD
- Substrate Properties: Chemical Substrate
- Substrate Type: Neuraminidase Substrate
- Target Enzyme: Neuraminidase, Sialidase
Specifications
| This kit contains 1000X NA-XTD™ Substrate, NA Sample Prep Buffer, NA-XTD™ Assay Buffer, NA-XTD™ Accelerator, and 10 detection microplates. Store all reagents at 2°C to 8°C. | |
| Influenza Neuraminidase Assay | |
| Neuraminidase Assay | |
| 96-well plate | |
| NA-XTD™, NovaBright™ | |
| NA-XTD™ | |
| Neuraminidase Substrate | |
| High-throughput Compatible |
| Enhanced Chemiluminescence | |
| Luminometer (Microplate) | |
| Liquid | |
| Less hazardous, Sustainable packaging | |
| 10 x 96 well plates | |
| Chemical Substrate | |
| Sialidase, Neuraminidase | |
| Enzyme Assay |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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