Cytiva Ni Sepharose™ 6 Fast Flow

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

• Designed for scaling up purification of histidine-tagged proteins.
• High protein binding capacity and minimal leakage of Ni2+ ions.
• As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
• Available in convenient prepacked HisPrep and HisTrap column formats.
• Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
• The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
• Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
• As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.