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Invitrogen™ Nitric Oxide Assay Kit
Measure nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.
Brand: Invitrogen™ EMSNO
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Description
Assay nitric oxide (NO) composition and total as separate values of nitrate (NO3-) and nitrite (NO2-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Nitric Oxide Assay Kit.
Additional Information and Online Ordering
Nitric oxide assay kit is for quantitative determination of nitrite (NO2-) and nitrate (NO3-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. The interaction of nitric oxide (NO) in a system is measured by the determination of both nitrate and nitrite concentrations in the sample. The relative levels of nitrite and nitrate can vary substantially, therefore the most accurate determination of total NO production requires measurement of both nitrate and nitrite.Target: Nitric oxide (NO) and relative levels of nitrite (NO2-) and nitrate (NO3-)
Format: Colorimetric stain (azo dye) for clear 96-well plates
Measurement: Absorbance at 540nm (± 20nm)
Assay Range: 0 to 100μM
Sensitivity: 0.222μM nitrite; 0.625μM nitrate
Precision: Dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)
Sample Types: Urine, saliva, plasma, serum and other biological fluids
Total Assay Time: 15 minutes (nitrite assay); 45 minutes (nitrate assay)
Sample Size: 50μL
Includes:
Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.
Specifications
| 2 x 96 tests |
For Research Use Only. Not for use in diagnostic procedures.