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Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Brand:  Invitrogen™ A44717

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Product Code. 16351358

  • 55.75 EUR / 10 reactions
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Description

Description

  • Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
  • Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
  • Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
  • Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
  • Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
  • Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.
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Specifications

Specifications

Protein Labeling Reagent
The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
A standard kit will label 10 mini gels or 10 mini gel-sized membranes
Dry Ice
Mini Precast Protein Gels, Midi Precast Protein Gels
Invitrogen™
• No-Stain activator, 200 μL, store at -5°C to -30°C
• No-Stain derivatizer, 200 μL, store at -5°C to -30°C
• No-Stain labeling buffer (20X), 2 x 5 mL, store at 15°C to 30°C
Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
10 minutes
Fluorescence
PVDF, Nitrocellulose
Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
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For Research Use Only. Not for use in diagnostic procedures.